Tag

Part:BBa_K4365019:Design

Designed by: Svetlana Iarovenko   Group: iGEM22_TU_Dresden   (2022-09-30)


Alpha mating factor signal peptide from Saccharomyces cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 23
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 23
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 23
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 23
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for yeast.


Source

Literature: https://academic.oup.com/femsre/article/24/1/45/525926?login=true

References